Effect of the capsid assembly modulator (CAM) ALG-000184 on HBsAg Levels in subjects with HBeAg positive chronic hepatitis B (CHB)

Background: ALG-000184 is a prodrug of ALG-001075, a novel, potent, pan-genotypic Class II CAM. CAMs are thought to have two mechanisms of action (MoA). The primary MoA affects pgRNA encapsidation resulting in inhibition of HBV DNA/RNA replication, as confirmed in CHB subjects receiving ALG-000184. The secondary MoA, which occurs at higher concentrations, regulates the establishment and replenishment of cccDNA, resulting in lowering of HBsAg, an effect that has not been reported to date with ALG- 000184. Method(s): ALG-000184-201 is a multi-part, multicenter, doubleblind, randomized, placebo-controlled study. In healthy volunteers (HVs), single doses up to 500 mg and multiple doses up to 250 mg were well tolerated with linear PK (Gane E., HBV TAG and APASL 2021). In treatment naive (TN) subjects with CHB, daily oral doses of 10-100 mg ALG-000184 for 28 days were well tolerated with linear PK and were associated with profound reductions of DNA/RNA regardless of HBeAg status or dose (Yuen MF, EASL 2022). Plasma exposures required to engage the secondary MoA are expected to be achieved at the 300 mg dose level. Data from a 300 mg cohort treated for 28 days are described here. Data from another ongoing cohort treated with 300 mg for 12 weeks will be presented at the conference. Result(s): Ten subjects were randomized to 300 mg ALG-000184 for 28 days and two to placebo. Two subjects randomized to ALG- 000184 were replaced due to missing data due to Covid-19 lockdown. Subjects were Asian, HBeAg positive, and genotype B or C. Mean baseline HBV DNA and RNA levels were 8.4 log10 IU/mL and 7.3 log10 copies/mL, respectively. One subject experienced a serious adverse event (AE) of pneumothorax>8 weeks after last dose which was considered unlikely related to study drug. No subjects prematurely discontinued study drug. All treatment emergent AEs were Grade <= 2 except for 4 Grade >= 3 alanine aminotransferase (ALT) elevations, which an independent ALT Flare Committee assessed as not related to study drug toxicity. PK was similar to HBeAg negative and HV cohorts following body weight adjustment. Subjects dosed with 300 mg ALG-000184 experienced mean declines of 4.0 log10 IU/mL and 2.6 log10 copies/mL in HBV DNA and RNA levels, respectively, at Day 28. Three of 7 evaluable subjects who received ALG-000184 had HBsAg declines>0.2 log10 IU/mL (0.23-0.78 log10 IU/mL). One subject receiving ALG-000184 had unquantifiable HBsAg throughout the study. Additionally, one HBeAg positive subject in a prior 100 mg cohort had plasma exposures equivalent to the 300 mg dose level and experienced a 0.5 log10 IU/mL HBsAg decline. Conclusion(s): In TN HBeAg positive CHB subjects, 300 mg ALG- 000184 for 28 days was well tolerated, exhibited predictable PK and resulted in rapid and substantial declines in HBV DNA and RNA. Notably, 3 of 7 evaluable subjects from this cohort experienced HBsAg declines of up to 0.78 log10 IU/mL. These data suggest that ALG-000184 can engage the secondary MoA of CAM II. Cohorts evaluating 300 mg over longer durations are planned or ongoing.

Speech Recognition Using Intelligent Piezoresistive Sensor Based on Polystyrene Sphere Microstructures

Rapid advances in wearable sensing technology have demonstrated unprecedented opportunities for artificial intelligence. In comparison with the traditional hand-held electrolarynx, a wearable and intelligent artificial throat with sound-sensing ability is a more comfortable and versatile method to assist disabled people with communication. Herein, a piezoresistive sensor with a novel configuration is demonstrated, which consists of polystyrene (PS) spheres as microstructures sandwiched between silver nanowires and reduced graphene oxide layers. In fact, changes in the device's conducting patterns are obtained by spay-coating the various weight ratios and sizes of the PS microspheres, which is a fast and convenient way to establish microstructures for improving sensitivity. The wearable artificial throat device also exhibits high sensitivity, fast response time, and ultralow intensity level detection. Moreover, the device's excellent mechanical-electrical performance allows it to detect subtle throat vibrations that can be converted into controllable sounds. In this case, an intelligent artificial throat is achieved by combining a deep learning algorithm with a highly flexible piezoresistive sensor to successfully recognize five different words (help, sick, patient, doctor, and COVID) with an accuracy exceeding 96%. Herein, new opportunities in voice control as well as other human-machine interface applications are opened.

Social ethical reflection on covid-19 epidemic from the perspective of caring ethics

COVID-19 has caused global concern and called for emergency response measures. In this continuous epidemic crisis, ethics has suffered unprecedented huge impact and challenges. It is the responsibility of contemporary ethics to reflect on the moral dilemma existing in itself and in the world. Therefore, ethics must stand the test and provide legitimate reasons for how to give full play to relevance and availability in this global crisis. The theory of rule ethics, which focuses on the realization of moral obligations and rights, is obviously unable to adapt to the response to the demand for ethical responsibility in special periods. The ability to take moral actions against others requires the intervention of "empathy" and "care" factors to realize the possibility of changing from heteronomy to self-discipline. Caring ethics emphasizes that "empathy" and "care" should be wisely regarded as an important value under the COVID-19 epidemic, so as to minimize the possible of the COVID-19 spread and its mutation and escalation in daily interpersonal communication, and create more meaningful ethical relationships with others by being responsible for and caring for others. At the same time, caring ethics further emphasizes that ignoring the cognition may lead to continuous psychological impact, making it difficult to reconstruct a harmonious social psychological situation. © 2023, Editorial department of Chinese Medical Ethics. All rights reserved.

Development of an Indirect ELISA Antibodies Detection Method on Tandem-epitope Peptide of African Swine Fever Virus p30 and p54 Proteins.

Here, we report the development of an indirect ELISA antibodies detection method for African swine fever virus (ASFV). Two purified monoclonal antibodies (mAbs) against ASFV p30 and p54 protein were used as targets and a phage-displayed 12-mer peptide library was used to conduct four rounds of biopanning to screen peptide epitopes, then amino acids GGG was used as a linker to synthesize tandem-epitope peptide of ASFV p30 and p54 protein which was used as coating antigen. The optimum reaction conditions of indirect ELISA were determined by chessboard titration, and clinical serum samples were used to evaluate the specificity, sensitivity, stability and conformity of this method. The biopanning experiment indicated that 146PAEPYTT152 was a core domain of the B cell linear epitope of p54 protein. The optimization results of ELISA reaction conditions showed that the tandem-epitope peptide coupled with ovalbumin (OVA) at N-terminal had low background of non-specific serum reaction. And the optimum reaction effect was obtained when the polypeptide antigen was coated with carbonate buffer in 2 mug.mL-1, the serum was diluted 100-fold with blocking solution (1% gelatin solution), and the HRP-antibody was diluted 5 000 times with 0.05% PBST solution. The cut-off value was determined to be 0.339. Furthermore, the results of specificity, sensitivity and stability tests showed that there is no cross-reaction in positive serum samples of classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), porcine parvovirus (PPV) and porcine pseudorabies virus (PRV), the detection limit of ASFV positive sera is 1:1 600, and the method had high repeatability. Finally, Total 320 swine serum samples were detected simultaneously by the present established method and commercial ASFV antibody detection kit. The results showed that the relative specificity and sensitivity of the two methods were 97.6% and 97.3%, respectively. And the coincidence rate was 97.5%. In conclusion, this method showed good specificity, sensitivity, repeatability and coincidence rate, that had the potential value of developing clinical diagnostic kit.Copyright © 2022 Editorial Board, Institute of Animal Science of the Chinese Academy of Agricultural Sciences. All rights reserved.

Social ethical reflection on covid-19 epidemic from the perspective of caring ethics

COVID-19 has caused global concern and called for emergency response measures. In this continuous epidemic crisis, ethics has suffered unprecedented huge impact and challenges. It is the responsibility of contemporary ethics to reflect on the moral dilemma existing in itself and in the world. Therefore, ethics must stand the test and provide legitimate reasons for how to give full play to relevance and availability in this global crisis. The theory of rule ethics, which focuses on the realization of moral obligations and rights, is obviously unable to adapt to the response to the demand for ethical responsibility in special periods. The ability to take moral actions against others requires the intervention of "empathy" and "care" factors to realize the possibility of changing from heteronomy to self-discipline. Caring ethics emphasizes that "empathy" and "care" should be wisely regarded as an important value under the COVID-19 epidemic, so as to minimize the possible of the COVID-19 spread and its mutation and escalation in daily interpersonal communication, and create more meaningful ethical relationships with others by being responsible for and caring for others. At the same time, caring ethics further emphasizes that ignoring the cognition may lead to continuous psychological impact, making it difficult to reconstruct a harmonious social psychological situation. © 2023, Editorial department of Chinese Medical Ethics. All rights reserved.

Implementation of radiotherapy in a tertiary hospital in Zhejiang province during the epidemic of COVID-19.

Objective: To explore the clinical practice of delivering radiotherapy during the outbreak of 2019 novel coronavirus disease(COVID-19). Method(s): During this epidemic period, available method including but not limited to: strict disinfection, body temperature monitoring and staff training of relevant knowledge, were used to ensure the safety of radiotherapy treatment. Statistical analysis was performed to study the relevant data including proportion of patients receiving radiotherapy for different purposes, time from scanning to the first time of radiation delivery and degree of satisfaction in the view of staffs and patients, respectively. Result(s): A total of 60 patients received radiation therapy in the department of radiotherapy of Zhejiang Provincial People's Hospital (2020-02-11). Compared with the same period in 2019 (after the Spring Festival), the total number of patients receiving radiotherapy was decreased from 72 to 60(83.3%). Among them, the number of patients receiving palliative radiation therapy decreased significantly, while the proportion of radical, preoperative and/or postoperative radiotherapy/radiochemotherapy did not significantly decrease. There was significant difference between two years (chi2=6.967, P<0.05). The median time for newly admitted patients to receive radiotherapy was two days, which was not significantly longer than the interval in 2019 (P>0.05). Staff and patients were generally satisfied with the current prevention measures. Conclusion(s): Using a variety of prevention and control method, and taking full account of medical safety and patient benefits, radiation-related activities can be carried out during the epidemic.Copyright © 2020 by the Chinese Medical Association.

Metformin regulates AMPK/SREBP-1 pathway and its clinical application

Metformin is one of the commonly used hypoglycemic drugs in clinical practice. In addition to hypoglycemia, there are a variety of medical biological values that have been constantly discovered and attracted much attention. In recent years, studies have shown that metformin through activation of AMPK inhibition of sterols regulating element binding protein 1 (SREBP-1) reduce lipid synthesis, in the treatment of liver steatosis, improve insulin sensitivity, prevention Metformin is one of the commonly used hypoglycemic drugs in clinical practice. In addition to hypoglycemia, there are a variety of medical biological values that have been constantly discovered and attracted much attention. In recent years, studies have shown that metformin through activation of AMPK inhibition of sterols regulating element binding protein 1 (SREBP-1) reduce lipid synthesis, in the treatment of liver steatosis, improve insulin sensitivity, prevention of atherosclerosis and cardiovascular dysfunction, tumor, polycystic ovary syndrome and adjuvant therapy of COVID-19 aspects play a role. Therefore, this article reviews the possible mechanism and clinical application of metformin in regulating glucose and lipid metabolism by inhibiting SREBP-1 through activating AMPK. Copyright © 2022 Chinese Journal of Clinical Pharmacology and Therapeutics. All rights reserved.

Resilience of urban public health system in cases of epidemic crisis

To effectively cope with urban public health crisis, the resilience of public health system is critical. With the successful prevention experience of COVID-19 epidemic in Wuhan as an example, grounded theoretical method was used to analyze concepts and behaviors of the government, communities, hospitals, media and Non-Governmental Organizations in the prevention and control of the epidemic. 10 main categories and 52 corresponding sub-categories, which reflected system resilience, were identified, including disease surveillance system, epidemic prevention resource management, and collaborative governance capabilities. It is found that public health system resilience can be divided into functional resilience, process resilience and integral resilience. A hierarchical structure model can be constructed to improve its resilience by means of interpretation structure modeling (ISM). And by integrating factors of multiple social subjects, including social epidemic prevention foundation, epidemic prevention technology and culture, and comprehensive governance of epidemic risks for gradual emergency preparedness, the resilience of the urban public health system can be upgraded. © 2022, Editorial Department of China Safety Science Journal. All rights reserved.

The COVID-19 crisis, Herd Immunity, and "Vaccine Apartheid" in the Age of Anthropocene

The coronavirus pandemic has led to millions of deaths around the world. In many countries, it has also exposed long-standing inequities and injustices in health care, income distribution, labour market practice, and social protection for the poor, women, indigenous peoples, and other marginalized segments of the population. The disproportionate casualties among vulnerable populations have also exposed predatory corporate practices, such as the refusal to share vaccine patents with low-income countries (LIC) in the Global South. World Health Organization (WHO) Director-General Tedros Adhanom Ghebreyesus has warned that this "vaccine apartheid" could lead to the further spread of more dangerous forms of virus variants, and that global solidarity and collaboration may be the only viable approach to current and future pandemics.(1) Scientists have long warned that the continued destruction of the environment and ecological diversity would lead to future waves of cross-species (zoonotic) viral pandemics, due to the elimination of "natural borders" that once existed between human and non-human species. In the last several decades alone, humanity has suffered from five major zoonotic pandemics: AIDS, SARS, MARS, Ebola, and COVID-19.(2) This Special Issue focuses on a select group of Asian countries in order to critically examine the impact of socio-legal inequities in state-centric policies upon domestic populations, including indigenous peoples, and to explore the possibility of international collaborative strategies for controlling the spread of deadly viruses and their variants in the coming years and decades, in Asia and beyond.

Molecular biological characteristics of the 2019 novel Coronavirus in Shijiazhuang

Objective To analyze the molecular epidemiological characteristics of the Corona virus disease 2019 (COVID‑19) cases in Shijiazhuang, which can reveal the origin of the outbreak and provide a scientific basis for COVID‑19 prevention and control. Methods From January 2 to January 8, 2021, a total of 404 samples from 170 COVID‑19 cases were collected from the Shijiazhuang Fifth Hospital. The consensus sequence of 2019 novel Coronavirus(2019‑nCoV) was obtained through multiplex polymerase chain reaction‑based sequencing. The sequences of 170 COVID‑19 cases were analyzed by the PANGOLIN, and the data were statistically analyzed by T‑test. Results Among the 404 COVID‑19 samples, a total of 356 samples obtained high quality genome sequences (>95%, 100×sequencing depth). The whole genome sequences of 170 COVID‑19 cases were obtained by eliminating repeated samples. All 170 sequences were recognized as lineage B1.1 using PANGOLIN. The number of single nucleotide polymorphism arrange from 18-22 and most of the single nucleotide polymorphism were synonymous variants. All of 170 genomes could be classified into 48 sub‑groups and most of the genomes were classified into 2 sub‑groups (66 and 31, respectively). Conclusions All cases in this study are likely originated from one imported case. The viruses have spread in the community for a long time and have mutated during the community transmission.

Alteration of right ventricular function after catheter ablation in patients with persistent atrial fibrillation and concomitant heart failure with preserved ejection fraction

Objective To investigate the alteration of right ventricular function after catheter ablation in patients with persistent atrial fibrillation (PAF) and concomitant heart failure with preserved ejection fraction (HFpEF). Methods The prospective observational study was performed in patients with HFpEF-PAF and undergoing first-time radiofrequency ablation procedures in the First Affiliated Hospital of Nanjing University between May to December 2019. Right ventricular functional parameters were measured before and 5 days, 1, 3, 6 and 12 months after the ablation by transthoracic echocardiography, respectively, including the right ventricular fractional area change (RVFAC). tricuspid annular plane systolic excursion(TAPSE), tricuspid annular diameter (TVAD). tricuspid annular peak systolic speed(TDI-S) and longitudinal strain of right ventricular free wall (RVFLS). Meanwhile, routine ECG and Holler recordings were performed at each follow-up time point. Results In this study, atrial fibrillation (AF) recurrence occurred in 4 patients at the 3rd month after ablation, and 7 patients failed to follow up due to the Covid-19. Finally, 19 patients were followed up for the evaluation of cardiac function after catheter ablation. Compared with pre-ablation, right ventricular structural and functional paramters (RVFAC, TAPSE, TVAD, TDI-S. RVFLS) improved significantly at all stages of follow-up(all P <0.05). Patients with atrial fibrillation recurrence had lower RVFLS and TDI-S at the baseline(P =0.039, P =0.019). Conclusions Right ventricular function could improve in HFpEF-PAF patients who maintain sinus rhythm after radiofrequency ablation. © 2022 ChinJUltrasonogr. All rights reserved.

Collection and quality characteristics of Coronavirus Disease 2019 convalescent plasma

Objective To explore the application and safety of apheresis technology in collection of Coronavirus Disease 2019 (COVID-19) convalescent plasma (CP), and to analyze the quality characteristics of the plasma. Methods The general data of COVID-19 convalescent plasma (CP) donors, including gender, age, date of discharge or release from medical isolation, were collected based on informed consent. After physical examination, the CP was collected by apheresis technology with plasma separator, inactivated with methylene blue, and determined for severe acute respiratory symptom Coronavirus 2 (SARS-CoV-2) nucleic acid and specific antibody (RBD-IgG) against SARS-CoV-2. Results The collection process went well, and no serious adverse events related to plasma collection were reported during or after the collection. The average age of COVID-19 CP donors was 38 years (n = 933). The distributions of blood groups A, B, AB and 0 in RhD (+) COVID-19 CP were 33. 4%, 29. 2%, 10% and 27. 2% respectively. The plasma donation date was 18 d from the discharge date in average. All the test results of SARS-CoV-2 nucleic acid in CP were negative, while the proportion of plasma samples at SARS-CoV-2 antibody titer of more than 1: 160 was 92. 60%. Conclusion Apheresis technology was safe and reliable. The COVID-19 CP contained high titer antibody. Large-scale collection and preparation of inactivated plasma against SARS-CoV-2 played an important role in the treatment of COVID-19.

[Research on COVID-19 vaccination strategies in Singapore].

As of December 31, 2021, Singapore reported that 4 758 601 had completed at least one dose of COVID-19 vaccination, 4 714 655 had completed two doses of COVID-19 vaccination, and 2 207 341 had received one booster shot of COVID-19 vaccine. This article analyses the current performance of COVID-19 vaccination in Singapore, interprets the content of Singapore's National Vaccination Programme, and systematically introduces specific measures of COVID-19 vaccination in Singapore, such as door-to-door vaccination, vaccination differentiated management, and self-payment of medical expenses for those who refuse to be vaccinated, to provide reference for the COVID-19 vaccination in China.

An undergraduate course for FOSS and with FOSS

Many universities have incorporated free and open-source software (FOSS) into undergraduate courses, though few research efforts have specifically investigated the interactions between undergraduates and real-world FOSS projects. In this paper, we present our experience of participating in the development of real-world FOSS projects, dealing with the challenges caused by COVID-19, and the feasibility of remote education with FOSS only. Our experience shows that undergraduates and FOSS projects can help each other well. Moreover, we summarize the strengths and weaknesses of the existing FOSS communities in attracting undergraduates and provide some suggestions. © 2021 Association for Computing Machinery.

The Impact of Social Media Use on Online Collective Action During China’s COVID-19 Pandemic Mitigation: A Social Identity Model of Collective Action (SIMCA) Perspective

The role of social media in fostering collective action in China is under constant debate, and the mechanism underlying the effects of social media use on collective action has not garnered sufficient scholarly attention. This study aims to investigate the (in)direct effects of attention to social media—administered by the governmental (gov) and nongovernmental sectors (nongov), respectively—for information about COVID-19 mitigation in China on intention to participate in online collective action (IPOCA). Findings from a survey suggest that attention to both social media (gov) and social media (nongov) directly predicted IPOCA. The indirect effect of attention to social media (gov) on IPOCA was significantly mediated by social identification. This study evidences the impact of social media on collective action in China and theoretically underpins its mechanisms through the social identity model of collective action © 2022 (Xin Zhao, Mengfei Guan, and Xinya Liang). Licensed under the Creative Commons Attribution Non-commercial No Derivatives (by-nc-nd). Available at http://ijoc.org

Innovative Contactless Palmprint Recognition System Based on Dual-Camera Alignment

Recently, contactless bimodal palmprint recognition technology has attracted increased attention due to the COVID-19 pandemic. Many dual-camera-based sensors have been proposed to capture palm vein and palmprint images synchronously. However, translations between captured palmprint and palm vein images differ depending on the distance between the hand and the sensors. To address this issue, we designed a low-cost method to align the bimodal palm regions for current dual-camera systems. In this study, we first implemented a contactless palm image acquisition device with a dual-camera module and a single-point time of flight (TOF) ranging sensor. Using this device, we collected a dataset named DCPD under different distances and light source intensities from 271 different palms. Then, a bimodal palm image alignment method is proposed based on the imaging and ranging models. After the system model is calibrated, the translation between the visible light and infrared light palm regions can be estimated quickly based on the palm distance. Finally, we designed a convolutional neural network (CNN) to effectively extract the fine- and coarse-grained palm features. Compared to widely used existing methods, the proposed networks achieved the lowest equal error rate (EER) on the Tongji, IITD, and DCPD datasets, and the average time cost of the system to perform one-time identification is approximately 0.15 s. The experimental results indicate that the proposed methods achieved high efficiency and comparable accuracy. In addition, the system's EER and rank-1 on the DCPD dataset were 0.304%and 98.66%, respectively. IEEE

The Right to Accessible COVID-19 Testing in the Post-Epidemic Period under the Urban-Rural Integration: Haishu District, Ningbo City, China

The reasonable distribution of COVID-19 testing facilities is a crucial public necessity to protect the civil right of health and the proper functioning of society in the post-epidemic period. However, most of the current COVID-19 testing facilities are in large hospitals in China, partially overlooking the COVID-19 testing needs of rural dwellers. This paper used shortest-path analysis and the improved potential model to measure the accessibility of current and potential COVID-19 testing facilities, superimposing this with the testing demands of residents, as calculated by the population demand index, so as to comprehensively evaluate the equity of the spatial allocation of the current and potential testing facilities, with a particular focus on Haishu District, Ningbo City, China. The results revealed that the overall accessibility of the current testing facilities in Haishu District was high, while the internal spatial differentiation was considerable. The comprehensive accessibility of testing facilities gradually declined from the downtown areas towards the rural areas. Moreover, roughly half of the rural population needing COVID-19 tests encountered hindrances due to poor access to testing agencies. However, after fully exploiting the potential testing facilities, the comprehensive accessibility of testing facilities was significantly improved, and the inequity in the accessibility to testing facilities was effectively alleviated, which significantly improved the equity of the allocation of testing facilities in Haishu District. The leveraging of current medical facilities to boost the number of testing facilities in rural areas could eliminate the disparity of resource distribution caused by urban and rural binary opposition, and could quickly identify external sources of COVID-19 in rural areas in the post-epidemic period. Moreover, efficient COVID-19 testing combined with the travel records of infection carriers can effectively identify unknown infection cases and obviate large-scale infection outbreaks.

Exploration of Online Teaching of Clinical Microbiology Based on PDCA Circulation

In order to “keep learning when class suspended” during the epidemic caused by coronavirus disease (COVID-19), the teaching team of clinical microbiology course makes full use of information technology to innovate the teaching mode and introduce the PDCA circulation in the field of quality management into online teaching. The teaching design is made from the four stages of plan, do, check and act. This method advocates that students should learn by themselves before class, actively discuss in class, expand thinking after class and show their learning achievements in various ways, so as to implement the “student-centered” concept. The teaching team can achieve online knowledge transfer on the basis of closed-loop integrity and continuity circulation, which provides a reference for improving the information technology teaching ability of the teaching team. © 2021, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

The diagnosis situation of the patients with COVID-19 in outpatients with fever in Wuha general hospital

Objective To analyze the epidemiological characteristics of febrile patients during the outbreak of the COVID-19 in Wuhan and explore its pathogenesis, so as to provide scientific basis and theoretical support for the prevention and control of the epidemic. Methods The data of 2 698 fever cases in fever clinic of general hospital were collected, including pneumonia cases and the positive cases of nucleic acid test of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and analysed by descriptive epidemiology. Results From January 1, 2020 to February 12, 2020, a total of 2 698 febrile patients were treated in the General outpatient department of Wuhan, including 683 (25.3%) patients with pneumo nia, in which 377 patients(55.2%) with virus pneumonia. Combined with imaging examination results and clinical manifestations, 289 cases were confirmed as COVID-19. However, 286 cases were confirmed as COVID-19 according to the results of nucleic acid detection. Compared with other age groups, the rates of people over 50 years old was higher in confirmed cases of COVID-19, especially for aged 60-69. Conclusion For viral pneumonia patients in fever clinic, if COVID-19 nucleic acid test is false negative or not detected, it should be determined according to imaging results and clinical manifestations. © 2021, Publication Centre of Anhui Medical University. All rights reserved.